求翻译成英文
1】.在经理的指导下按时按量完成80家企业的做账报税工作2】.吃苦耐劳，有强烈的责任心，能适应较高的工作强度3】. 参与***专项审计凭证查询环节4】,积极主动，有高度学习能力，掌握各种办公室文员工作5】.参与组织有关电子商务知识讲座，负责引导师生有秩序地进退场6】. 参加校内“网页设计大赛”，获得团队第三名7】. 参与组织工商管理系“爱生活，I 微笑”创意T恤设计大赛，担任展示入围决赛T恤的模特8】. 参与组织工商管理系“母亲节挑战杯”活动，负责“滴水之恩”游戏环节，以及负责跟拍、记录一组决赛团队的社会实践过程
1 】in the manager under the guidance of the timely completion of 80 enterprises, accounting for tax filing 2 】hard-working, have a strong sense of responsibility, able to work with high intensity 3 】participate in * * * special audit certificate access link 4 】proactive, highly learning ability, to master a variety of office article member 5 】participating organizations related to electronic commerce knowledge lectures, responsible for the guidance of teachers and students in order to advance and retreat 6 】to participate in the "Webpage design competition", won the team third 7 】participate in the organization of industrial and commercial administration department "love life, I smile" creative t-shirt design contest, as shown in the final model into T-shirt 8 】participating in the Department of business administration "mother's Day" Challenge Cup "activities, responsible for you" game, and is responsible for the process of social practice with the racket, recording a group of final team
Bombyx mori Densovirus (Bombyx two Mori bidensovirus, BmBDV) is the first to have its encoding DNA polymerase invertebrate viruses, its genome consists of two kinds of ssDNA (VD1 and VD2). The virus specific recognition of the host cell is a columnar epithelial cells of the midgut of silkworm, silkworm pathogens causing chronic is concentrated nuclear conditions, its replication mechanism is not clear. This paper mainly in the BmN and Sf9 insect cells, the full-length genome fragments of the virus of two kinds of linear co transfection of these two kinds of insect cells, and the transfected cells of silkworm larvae feeding back a sense of susceptible strain, respectively from the viral gene transcription and expression in insect cells, to detection of transfected to produce infectious virus particles, and thus to replication of the virus to speculate and study. 1, the linear co transfected cells were infected by testing and training methods to explore the enzyme the virus genome cloning plasmid pT-VD1 and pUC-VD2, precipitation collection with a specific enzyme cutting site genome fragments VD1 and VD2 using alcohol, linearization were transfected into BmN cells, the cultured and continuous cultivation in two ways, the cultured for 20 days. (1) to collect the cells and supernatant medium as template, PCR was detected, amplified bands of virus gene into cultured in continuous culture and passage of cell mass. (2) total protein extraction cells and supernatant were detected in Western blot, cultured cell clusters in the cross to the bands associated with viral proteins. (3) after transfected into cultured BmN cells were blown, indirect immunofluorescence, positive reaction was localized in the nucleus. Save the virus back 2, the BmN cells transfected BmN cell mass detection collected respectively, feed and susceptible strains of Bombyx mori in 306 of the two instar silkworm larvae, feeding to five age fourth days. (1) from the midgut of silkworm genome, as detected by PCR in BmN cells, feeding the genome can be amplified to stripe virus gene. (2) to extract the total protein of Bombyx mori midgut, through the Western blot detection, hybridization and virus gene expression bands relevant only when total protein feeding cell clusters in. 3, the linear co transfected Sf9 cell infection detection will digestion collection of genomic fragments VD1 and VD2, linear co transfection of BmN and Sf9 cells were cultured for 20 days. (1) the genome of transfected cells was, by demethylation treatment, PCR detection, can be amplified to stripe virus gene in BmN genome. (2) the total protein in transfected cells by Western extraction, detection of blot hybridization, the strip is associated with viral protein can only in the total protein in BmN cell. 4, to save the virus transfected Sf9 cells to sense the transfected collection of BmN and Sf9 cells, respectively, feed and susceptible strains of Bombyx mori Jingsong two instar silkworm larvae, feeding to five age fourth days. (1) extract the midgut tissues of the genome, as detected by PCR, only in the feed and strip virus gene amplified from BmN cells group. (2) to extract the total protein of Bombyx mori midgut tissues by Western blot hybridization, detection, the strip is associated with viral protein can only in the total protein of BmN cells in feed. Therefore, the method of genome segment linearization BmBDV were transfected into BmN cells under in vitro conditions, can save the infectious virus particles. Keywords: Silkworm two densonucleosis virus, BmN cells, Sf9 cells, linear co transfection, virus rescue
I'm a very special examinee. I realize my merits and dismerits very well. My disadvantages are :1. I'm very bad at English listening and speaking and I can hardly comunicate with others in English, so I hope I can be allowed to answer questions in Chines.2,I'm a student from other subject, so the basis of my specialized course is weak. But this doesn't matter, I can spend much more time to make up it . My advantages are: 1. Because I have worked for a period time on society, so I can cope with all things in study and daily life. 2. As I have quit my jio, so I go back school not for kill time but for study. There is no doubt that I will study very hard.I sinserely hope that I can be allowed to answer questions in Chinese.
sorry啊 感觉你的中文表达也有点儿混乱 暂时只能帮忙翻成这样了……
I am a special examinee, I know, my poor : 1,. English listening is very poor, the basic will not hear. Therefore, I hope you can use Chinese to answer some question. 2. I is a professional, so, I started the foundation is very bad, but this does not affect, I basic capability is very strong, I can use another ten times longer to fill. To try to prove my point. But I also have a great advantage:1, because of work, so can the very good processing all kinds of learning and in life. 2. I'm not back dawdle, I came back to the real learning. I will harder than others, and this is beyond doubt. Otherwise I won't quit. I request, can use Chinese to answer some question.
I was a special candidate, I know, poor me: 1,. English listening and speaking is very poor, not heard. So, I hope to answer some questions in Chinese. 2. I boast professional, so first I basis is very poor, but this does not impact my basic capacity is very strong, I can use the other 10 times the time to complement. First proved my point. But I also have a lot of advantages: 1, because of work, so can handle all kind of good things and life. 2. I came back not mess around, I am coming back real learning. I will seriously than others, it is beyond doubt. Or I will not resign. In the time I requested, can answer some questions in Chinese.
或者
I am a special examinee, I knew, I miss aspect: 1.English heard very badly, basic cannot hear.Therefore, I hoped can use Chinese to answer certain questions.2。I praise the specialty, therefore, just started my foundation to be very bad, but this did not affect, my foundational ability was very strong, I might use others ten time of time to make up.The preliminary test has proven my this point.But I also have the very big merit: 1, because has worked, therefore can in very good processing each kind of study and the life matter.2。I am not come back to drift along, I am come back to study truly.I certainly compared to others earnest, this is without a doubt.Otherwise I do not resign.I requested in the time that, can use Chinese to answer certain questions
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